A position for a Research Technician Associate is available in the Division of Cardiovascular Medicine at the University of Michigan, in a laboratory studying diseases of the vessels, the heart, the lungs, and the brain. The successful candidate will become a member of a collaborative and inter-disciplinary research lab involved in the identification and functional analysis of molecular factors contributing to inflammation and vascular disease. The position involves utilization of molecular and cell biology methods for cellular immunology and vascular biology experiments, using cell culture systems, animal models including microsurgery, and patient samples.
- Selected individual will spend a majority of their time managing and genotyping genetically altered mice including the management of a breeding program.
- Establish and maintain cell culture lines, and manipulate cell cultures with drugs or other stimuli to obtain cell lysates for expression analysis; prepare samples from cell cultures or tissues for gene expression and protein analysis.
- Conduct molecular and protein assays (electrophoresis, PCR, real-time PCR, immunoblotting, Western blotting, immunopreciptation for protein interactions, ChIP assays, southern blotting).
- Perform microsurgical procedures in mouse models. Harvest and analyze tissues for flow cytometry, morphologic, and immunohistochemical assessment.
- Maintain detailed and accurate records of experiments; inventory of materials; analyze data for publication under the direction of the PI; participate in manuscript preparation.
- Catalogue experimental samples, perform flow cytometry, isolate DNA, RNA, and protein from blood samples, perform quality control checks, and quantify.
- Coordinate and maintain compliance with regulatory protocol including but not limited to UCUCA and OSEH
- Attend scheduled laboratory meetings.
The applicant should be highly motivated, reliable, and be able to work well with different members of the laboratory team, ranging from students to staff to faculty. Experience is required with cell culture methods, molecular biology and basic protein assays as described above. Proficiency with stereotactic/microsurgical technique in rodents is also required.
Experience genotyping mice, and willingness to help manage mouse colony of genetically altered mice including management of breeding program. Exceptional record-keeping skills is essential.
A minimum of a Bachelor's degree is required for this position
Experience with animal models of vascular diseases is a plus. A background in vascular biology or cardiovascular disease is preferred.
Hours: 40 hours
Shift: Days / Monday - Friday / Hours negotiable and based on research needs
Benefits: Medical School vacation/sick eligible
We, the staff and faculty of the U-M Cardiovascular Center (CVC) team, are committed to advancing medicine and serving humanity through living and teaching our core values of Respect and Compassion; Collaboration; Innovation; and Commitment to Excellence.
Each CVC employee is expected to understand and demonstrate that in every interaction we represent our entire organization in the care we provide and in the courtesies we extend to patients, families, and each respective team member. The CVC is dedicated to partnering with patients and families to deliver the safest and highest quality of health care. Applicants are expected to review the following PowerPoint presentation which provides an overview of the Cardiovascular Center's philosophy and culture:
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The University of Michigan Health System improves the health of patients, populations and communities through excellence in education, patient care, community service, research and technology development, and through leadership activities in Michigan, nationally and internationally. Our mission is guided by our Strategic Principles and has three critical components; patient care, education and research that together enhance our contribution to society.
Job openings are posted for a minimum of seven calendar days. This job may be removed from posting boards and filled anytime after the minimum posting period has ended.
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The University of Michigan is an equal opportunity/affirmative action employer.