The Department of Molecular & Integrative Physiology is looking for a temporary Research Lab Technician in a lab that focuses on the CNS regulation of energy and glucose homeostasis, body weight, and feeding behavior using mutant mouse models. The qualified applicant will assist and/or cover the duties of my existing lab manager during their absence for medical reasons. Expected hours are a minimum of 20 hr/wk, and possibly as much as 40 hr/wk over the coming 6 to 7 months.
Experimental responsibilities (80% effort) will include but are not limited to:
- Maintain multiple mouse colonies including breeding, tagging and PCR genotyping.
- Small animal handling, injections, perfusions and dissections. Weekly or daily observation and recording of data such as food and water consumption, body weight, and body composition by NMR in experimental animals.
- Other potential duties include: Tissue sectioning; fresh frozen, perfused, or paraffin-embedded. Perfusion and routine histological staining, immunohistochemistry and in situ hybridization. DNA preparation from bacterial cultures, plasmid and genomic cloning. RNA and DNA preparation from mouse tissues and RT-qPCR reactions.
Non-experimental responsibilities (20% effort) include:
- Administration and compliance with radiation safety and biosafety regulations and completing lab documentation. (5%)
- General lab organization, maintenance of freezer, liquid N2, chemical and equipment inventories. (5%)
- Ordering of supplies and oversight of lab equipment. (5%)
- Orientation of new personnel and trainees to the lab environment. (5%)
B.S. degree or equivalent in biological sciences or related field; neuroscience background highly desirable. At least one year previous laboratory experience handling mice, maintaining mouse breeding colonies and genotyping by PCR are essential. The successful candidate must already have taken the required training for mouse handling and procedures offered by the Unit for Lab Animal Medicine so that there will be no delay in starting work. Other desired experience includes performing immunohistochemistry, in situ hybridization and DNA cloning. This position requires a focused and highly motivated candidate who can initiate and complete assigned tasks independently. The applicant must be able to follow detailed written or verbal instructions from the PI and other senior researchers in the Lab.
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