How to Apply
Interested applicants should formally apply for the position by clicking the "Apply Now" button located in the upper right hand corner of this posting and uploading their curriculum vitae with a cover letter. These materials should address your specific interest in the position and outline your skills and experience that directly relates to this position.
The Life Sciences Institute (LSI) is a multi-disciplinary research institute at the University of Michigan, strategically located as a bridge between the medical campus and the central-campus schools and colleges. Our mission is to advance the understanding of the fundamental processes of life, and to pioneer new ideas to improve human health.
The LSI’s world-class faculty members hold joint appointments at the LSI, where their labs are located, and a variety of schools and colleges across the university. They bring broad and diverse expertise — ranging from cell biology to medicine, from chemistry to structural biology. The institute’s philosophy is to provide our faculty and their teams with superior scientific tools and administrative support, allowing them to spend more time focused on what really matters — their research.
The institute’s administrative staff supports all aspects of unit operations including facilities, IT, human resources, finance, research administration, academic programs, communications and development.
In addition to faculty labs, the LSI houses several core technology labs that support the research enterprise of the entire university, including a high-throughput screening center (Center for Chemical Genomics), a comprehensive cloning and macromolecular X-ray crystallography facility (Center for Structural Biology), and a state-of-the-art cryo-electron microscopy facility. The LSI also plays a key role in administering campus-wide programs like the U-M Program in Chemical Biology, Center for the Discovery of New Medicines and Michigan Life Sciences Fellows.
The LSI is committed to the principle that fostering diversity, equity and inclusion within the institute — and in the next generation of scientific leaders — is inseparable from our commitment to scientific excellence and achievement.
The Center for Structural Biology (CSB), within the Life Sciences Institute at the University of Michigan (http://www.lsi.umich.edu/centers/center-for-structural-biology), seeks a Research Laboratory Technician Senior to advance the research objectives of principal investigators in a wide variety of basic science fields and drug development.
The CSB includes:
- A high-throughput cloning and expression laboratory for protein engineering
- Protein expression and purification facilities for small- and large-scale protein production
- Macromolecular crystallization and crystallography laboratories for solving crystal structures of biological molecules
- X-ray facilities with membership access to high energy synchotron radiation (LS-CAT beamlines at the Advanced Photon Source, Argonne National Lab)
- The development of an RNA production facility
The technician will work in the high-throughput cloning and expression laboratory under the direction of W. Clay Brown, Ph.D, and work in collaboration with members of the CSB and researchers who use the facilities.
The successful candidate will apply their energy and expertise to cutting edge science and drug discovery by:
- Constructing gene fragments by PCR for cloning, screen clones for positive inserts
- In multi-parallel format, cloning gene fragments using LIC or Gibson assembly
- Running small-scale multi-well trials for evaluation of protein production
- Assisting in eukaryotic cell culture (insect and mammalian) and large scale protein production using a stirred-tank bioreactor
- Doing small-scale protein purification from bacterial and eukaryotic (insect and mammalian) cell cultures using multi-well formats
- Assisting in large scale protein purification
- Maintaining laboratory stocks of buffers and competent cells
- Maintaining and order laboratory supplies and chemical inventories
- Initially working under the supervision of current CSB staff with the expectation of developing independence
- Aiding in coordinating projects on schedule
- Maintaining clear laboratory notebooks, perform quantitative analyses, and communicate findings through reports and summaries.
- Providing detailed documentation so experiments can be replicated
- Following laboratory best practices for safety and quality
- Bachelor’s degree in molecular biology, cell biology, biochemistry or a closely related field
- Minimum of 2 years hands-on experience working in a laboratory setting
- Minimum of 1 year experience performing aseptic and basic microbiology techniques such as transformation, plating and liquid cultures
- Minimum of 1 year experience with DNA preparation, mini and maxi
- Minimum of 1 year experience performing molecular techniques including PCR, primer design, and molecular cloning
- Minimum of six months experience with eukaryotic cell culture
- Minimum of six months experience with protein purification techniques
- High degree of motivation and the ability to think independently and work collaboratively
- Excellent verbal and written communication skills
Evidence of qualifications is provided by previous employment, references and performance evaluations
- Bachelor's or Master’s degree in biology, biochemistry or related field with 3+ years of previous experimental laboratory research experience
- Knowledge of protein characterization and biophysical techniques
The University of Michigan conducts background checks on all job candidates upon acceptance of a contingent offer and may use a third party administrator to conduct background checks. Background checks will be performed in compliance with the Fair Credit Reporting Act.
Job openings are posted for a minimum of seven calendar days. This job may be removed from posting boards and filled anytime after the minimum posting period has ended.
U-M EEO/AA Statement
The University of Michigan is an equal opportunity/affirmative action employer.