A laboratory in the Division of Rheumatology is seeking a full-time technician to participate in translational studies of innate immune function in autoimmune and thrombotic diseases, including lupus, systemic vasculitis, and antiphospholipid syndrome (APS). The laboratory uses cutting-edge methods to phenotype patient cells, while leveraging novel animal models for the assessment of potential therapeutic targets. All of this work is with the goal of identifying more personalized approaches to the treatment of our patients.
Assist senior laboratory personnel with ongoing projects, utilizing techniques such as ELISA, live-cell immunofluorescence microscopy, intravital microscopy, imaging flow cytometry, western blotting, and quantitative PCR. Perform cell and plasma isolation, store samples, and keep meticulous inventories. Troubleshoot problems as they arise, and suggest ways to streamline and improve existing protocols. Past experience suggests that highly-motivated technicians can also work on their own project in parallel to their partnership with senior lab personnel.
Desire and ability to work with a multidisciplinary team of physicians, students, and professional scientists. Bachelor's degree in a scientific field, and 1-2 years of research laboratory experience. Proficiency with standard software such as Microsoft Excel and GraphPad Prism, and strong writing and communication skills. Motivation to publish results. Professional demeanor and strong interpersonal skills.
Experience handling and genotyping mice. Experience with in vitro cell culture. Experience with ELISA and other plate-based assays.
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