A molecular neuroscience laboratory is recruiting for this position. The laboratory studies the fundamental mechanisms of human neuronal development during stem cell differentiation as it relates to the study of mechanisms of neuropsychiatric disorders.
The successful applicant will play key supervisory roles in research collaborations within the University of Michigan Medical School as well as with groups at other universities. Daily laboratory supervision of undergraduates and graduate students is required as well as excellent skills in laboratory organization, data analysis and scientific presentation skills including manuscript preparation.
Bachelor's degree and 5-6 years of related experince.
This position requires a deep expertise in the generation, maintenance and quality control assays for human embryonic stem cells and human induced pluripotent stem cells that is found with 4 to 5 years of experience. Specifically, experience with genotyping of stem cells using SNP-Chip analysis, embryoid body formation and immunocytochemistry is necessary. In addition, the job requires a knowledge of neuronal differentiation strategies that is obtained with 4 to 5 years of experience with both overexpression and small molecule-driven neuronal differentiation. A sophisticated molecular biological background in terms of expression vector construction, gene expression assays for RNA (qRT-PCR and in situ hybridization), proteins (western blotting and immunocytochemistry) and chromatin modifications (ChIP) is also required. Finally, a practical working knowledge of RNAseq library generation as well as bioinformatic strategies to analyze gene expression is also necessary.
PhD in a biological science or MD, with 4 to 5 years of research experience in molecular biology, cell culture and neuroscience. We are seeking an industrious, well-organized researcher. This position requires 3-4 years of postdoctoral laboratory experience in the culture and neuronal differentiation of human embryonic and human induced pluripotent stem cells. Experience with both excitatory and inhibitory neurons derived from stem cells is needed. Molecular biological experience in the generation of RNA and RNAseq libraries is also required. The position additionally requires 3-4 years of bioinformatics training in RNAseq analysis for the purpose of differential expression analysis and detailed gene ontology. The successful applicant will have expertise in the generation of expression vectors, CRISPR targeting vectors and stem cell mutagenesis for the development of in vitro models of human neurological and psychiatric disorders. Finally, expertise in the use of genetically encoded calcium indicators for the study of networked human neuronal cultures and the analysis of this network activity at a functional genomic analysis is essential.
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Job openings are posted for a minimum of seven calendar days. This job may be removed from posting boards and filled anytime after the minimum posting period has ended.
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The University of Michigan is an equal opportunity/affirmative action employer.