The Michigan Center for Translational Pathology is looking for an energetic, highly motivated individual to join our cancer biology and therapeutics group as a Research Laboratory Specialist Associate. The successful candidate must be capable of thriving in a fast-paced laboratory environment. The candidate will work as part of a research team to functionally interrogate epigenetic genes that activate essential oncogenic pathways in hormone-driven cancers, and assess their therapeutic druggability using in vitro and in vivo models. The individual should be familiar with routine molecular biology techniques and have an educational background in biomedical sciences or related subjects. The candidate will work alongside MCTP trainees (i.e., graduate students and post-docs) and will be directly supervised by a Principal Investigator or faculty member. The candidate will be provided thorough training in all tasks required as part of the job and will be involved in collaborative and intellectually-engaging translational research. Detailed descriptions of expected duties are included under “Responsibilities.”
Michigan Medicine improves the health of patients, populations and communities through excellence in education, patient care, community service, research and technology development, and through leadership activities in Michigan, nationally and internationally. Our mission is guided by our Strategic Principles and has three critical components; patient care, education and research that together enhance our contribution to society.
- Routine monolayer cell culture maintenance: Culture adherent and suspension cells, plate cells for experiments, generate cryo-frozen stocks and maintain detailed record of the cell line bank.
- Generation and maintenance of 3D organoids: Virally-engineer normal and cancerous prostate organoids from mouse and human donors to interrogate the functional role of chromatin and epigenetic driver oncogenes in transformation and metastatic progression.
- In vitro characterization of drug lead compounds in cancer cell lines: Calculate of IC50, perform proliferation assays etc.
- Functional assays: Perform protein and RNA extraction from cells and tumor tissue, western blot, co-immunoprecipitation, qPCR, ELISA assay, gDNA extraction, T7 endonuclease assay, bacterial transformation and plasmid propagation, DNA cloning and point mutagenesis.
- Generation of CRISPR-Cas9 models: Engineer cancer cells using the CRISPR-Cas9 technique to either knock-out or knock-in genes-of-interest and generate clonal lines. Perform phenotypic characterization of successfully-engineered clones.
- In vivo work: restrain SCID, FVB, and NSG mice, ear tagging, tumor measuring, drug preparation; Subcutaneous, intraperitoneal, and intra-cardiac injections of cells to generate tumor models. Bioluminescence Imaging, and tumor necropsy.
- Other wet laboratory techniques or assays that may be required depending on how the project progresses.
- Basic cell and molecular biology background.
- Basic molecular biology experimentation skills.
- Interest in learning basic bioinformatics skills.
- Strong organizational and multi-tasking skills.
- Excellent documentation skills for research results.
- Excellent teamwork and communication skills.
- Excellent time management skills.
- Ability and willingness to learn and implement novel experimental techniques.
- Knowledge and understanding of laboratory safety and ability to work carefully with potentially harmful agents.
- Some research lab experience.
- Bachelor's degree in biomedical sciences or related subjects, Master's preferred.
- 1 to 3 years related experience.
Michigan Medicine conducts background screening and pre-employment drug testing on job candidates upon acceptance of a contingent job offer and may use a third party administrator to conduct background screenings. Background screenings are performed in compliance with the Fair Credit Report Act. Pre-employment drug testing applies to all selected candidates, including new or additional faculty and staff appointments, as well as transfers from other U-M campuses.
Job openings are posted for a minimum of seven calendar days. The review and selection process may begin as early as the eighth day after posting. This opening may be removed from posting boards and filled anytime after the minimum posting period has ended.
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